Department of Obstetrics and gynecology, Helwan University, Helwan, Egypt
 Research Article   
                                        
                                                                                Laser assisted ALU-qPCR to evaluate the association between cell-free DNA in human follicular fluid with oocyte and embryo quality in assisted reproductive techniques 
                                                                                Author(s): Rabab M. Sorour, Mohamed A. El Noury, Osama Fekry, Sondos Salem, Tamer Taha and Karim Abdelsalam*             
                                        
                                                                                
                                 Background: Cell-free DNA (cfDNA)  has the potential to provide non-invasive diagnostic assays and practical  biomarkers for predicting the outcome of assisted reproductive technology (ART)  procedures. Aim of the study: To  investigate the association between cf-DNA concentration in individual  follicular fluid and oocyte maturity and embryo quality in patients undergoing  Intra Cytoplasmic Sperm injection (ICSI).
Patients and methods: This prospective  observational clinical study was done at Ain Shams University, A maternity  hospital (IVF Unit), and a private ICSI center from May 2019 to June 2020. Our  study used the ALU-QPCR technique to accurately measure the cell-free DNA  (cfDNA) level in 35 follicular fluid samples from 15 patients undergoing ICSI.
Results: Our results displayed  the range of.. Read More»