Abstract
Author(s): Shimaa Sabry Abd El Ghany*, Nermine Helmy Mahmoud, Azza Abd El Rahman Saab, Mohamed Ahmed Hassan El Kadi and Doaa Mostafa Awad
Background: Pre-eclampsia (PE) is a fatal disease for both mother and fetus diagnosed by criteria of ACOG 2017. Gene polymorphisms as renalase gene rs10887800 and rs2576178 are proposed for early prediction and prognosis of PE. This study aimed at assessing the relation between renalase gene polymorphism and development of PE as well as the severity of the disease.
Subjects and Methods: This case-control study included 40females (group I) with PE further subdivided into group Ia 20 non severe PE, group Ib 20 severe PE and group II 20 healthy controls. Renalase gene polymorphisms assessed by PCR-RFLP and confirmed by gene sequencing.
Results: Our results found Renalase rs10887800 homozygous GG& heterozygous AG genotype in 67.5% of PE females compared with 20% in controls, while AA(wild genotype) was found in 32.5 % of PE females and in 80% of controls. A highly significant statistical difference was seen rs10887800 genotype distribution (Χ2 = 12.047; p =0.001) and odds ratio was 8.308 with 95% confidence interval (2.31 - 29.88).Similarly, rs2576178 homozygous GG& heterozygous AG genotype were found in 72.5% of PE females compared to 15% in controls, while AA wild genotype was found in 27.5 % of PE females and in 85% in controls. A highly significant difference found in rs2576178 genotype distribution (Χ2 = 17.712; p = 0.001), the odds ratio was 14.94 with 95% CI (3.65 – 61.19). An increased frequency of G allele of both rs10887800 and rs2576178 was observed in PE females compared to controls. Results of the study were confirmed by Gene sequencing.
Conclusions: Our study highlights the role of renalase gene polymorphism both rs10887800 and rs2576178 (homo and heterozygous) in PE being strongly linked to occurrence of non-severe & severe PE patients. Thus suggesting a promising tool for PE assessment and predicting disease severity.