Abstract
Author(s): Alaa Ali Shaheen*, Kiaser Abdulsajjad M. Hussain, Alaa Hussein Al-Safy
In this research, a green nanocomposite was meticulously prepared and characterized. Its biological effectiveness was thoroughly evaluated before and after loading it with metformin in female white rats induced with polycystic ovary syndrome (PCOS). The green nanocomposite, derived from fresh, soft asparagus stalks, underwent rigorous preparation and was carefully characterized. The nanocomposite was meticulously created by mixing 20% fresh asparagus plant with 80% silver nitrate solution (AgNO3), and then the treatment was loaded onto it and diagnosed. Rigorous analysis involving Fourier transform infrared diffraction spectroscopy (FT-IR) and Atomic Force Microscope (AFM) images contributed to unveiling significant changes in the nanocomposite surface loaded with the treatment, all within nanoscale sizes and dimensions consistent with the results of the infrared spectrum (FT-IR). The therapeutic treatments' impact on immune parameters was comprehensively studied using 25 female white rats divided into five groups. The study conclusively indicated a significant increase (P<0.05) in the levels of cytokines IL-10 and IL-17 in the PCOS induction group (G2) compared to the control group (G1). Additionally, a significant decrease (P<0.05) in the levels of cytokine IL-10 in all treatment groups (G3-Meto., Asp.NPs-G4, G5- Asp.NPs+Meto.) compared to the PCOS induction group (G2), and a decrease (P<0.05) in the level of IL-17 in all treatment groups (G3-Meto., Asp.NPs-G4, G5- Asp.NPs+Meto.) compared with the PCOS induction group (G2) were noted. The evidence was compelling and led to the conclusion that the nano-asparagine complex was successfully prepared and displayed superior effectiveness in treating polycystic ovary syndrome compared to metformin. Furthermore, when metformin is loaded onto the nano-asparagine complex, its therapeutic effectiveness increases, reducing treatment time, side effects, and financial costs associated with repeated treatment.