Author(s): HUBERT HURAS1, RERO? ALFRED1, KRZYSZTOF RYTLEWSKI1, ANNA WÓJTOWICZ1,KATARZYNA KU?MIERSKA1, ROBERT JACH2, MAREK SAJEWICZ1
Introduction. One of the factors leading to the occurrence of preterm labor is premature ruptureof membranes. For proper functioning of the amniotic sac is needed proper stabilization ofthe extracellular matrix of fetal membranes. Premature rupture of membranes combined withintrauterine infection is associated with a significant increase in the average level of MMP-1. This increase is due to the presence in amniotic fluid inflammatory mediators, particularlyIL-1-beta, TNF-alpha, having the capacity deterioration in gene expression of MMP-1.Aim. The aim of this study was to determine the levels of TNF-alpha and IL-1-beta, whichclearly indicate the occurrence of premature rupture of membranes. Materials and methods.The study was a prospective, controlled study of 40 pregnant womenhospitalized due to premature rupture of membranes and control group of 40 pregnant womendelivered on time in the Department of Obstetrics and Perinatology, Jagiellonian Universityin the period from January 1 to December 30, 2009 year. Serum level of TNF-alpha andinterleukin-1-beta was mesuered twice during hospitalization and during labour in study groupand in control group only during labour.Results. The study showed that TNF-alpha values above 0.856 pg/ml and interleukin-1-betathan 0.412 pg/ml, clearly indicate the occurrence of premature rupture of membranes.